Master Thesis Project

Establishing a platform for generation of E.coli based protein expression systems

NorthX Biologics is a Contract Development and Manufacturing Organization that offers services and support in the development and manufacturing of biologics used as vaccines, gene therapy and other advanced applications. We have a long tradition of pharmaceutical manufacturing and have been manufacturing biologics to GMP since 1992. We are recognised as a national innovation hub for advanced therapeutics and have expanded our development capabilities. This includes a new development organisation with cutting edge laboratories and technologies.

Are you a driven and curious student nearing the end of your Master’s program in Molecular Biology, Biotechnology, or a related field? Do you enjoy turning theory into practice, and are you motivated by challenges in protein production for real-world applications? If so, this thesis project at NorthX Biologics could be the perfect match.

Project Description

The goal of this Master’s thesis is to design and validate a structured platform for screening and establishing of E. coli-based protein expression systems protein therapeutics. The project incorporates theoretical analysis and planning followed by hands on lab work to demonstrate the capability of the platform

A preliminary project layout could be:

1.       Theoretical Evaluation

-          Comparative analysis of E. coli strains, expression vectors, and culture formats

-          Selection of suitable analytical methods for expression analysis

-          Literature-based assessment of key parameters affecting yield and quality

2.       Proof-of-Concept Laboratory Work

-          Application of the designed workflow using selected model proteins

-          Hands-on work from DNA sequence to expression-ready E. coli cell lines

-          Evaluation of expression yield and protein quality

The ideal outcome is an effective platform that starts with a theoretical protein or DNA sequence and results in a transformed E. coli strain capable of producing the target protein at yields and quality levels suitable for manufacturing. During the thesis work you will gain practical experience in molecular biology methods, microbial bioprocessing in different scales, protein isolation and analysis. The project is located at NorthX Innovation laboratory in Matfors and is expected to have approximately 20 weeks’ duration and the start date is flexible from the beginning of September.

Who Are We Looking For?

We are looking for a highly motivated and self-driven Master student who:

-          Has a theoretical background in biotechnology, molecular biology, and protein expression

-          Excels in finding, analyzing, and applying scientific information

-          Has a keen interest in molecular biology and microbiology techniques

-          Previous experience with E. coli expression systems, recombinant protein production, or analytical methods (e.g., SDS-PAGE, Western blot) is a plus but not mandatory.

-          Holds a positive attitude and a curious mind

What We Offer

-          Supervision by experienced scientists within an industrial biotechnology setting

-          Modern laboratory facilities and equipment

-          A project contributing directly to NorthX Biologics' innovation pipeline

-          An opportunity to make an impact in the field of advanced biologics manufacturing

For further information, please contact:

Agnes Zimmer Ph.D . Scientist                                               agnes.zimmer@nxbio.com

Ola Tuvesson, Chief Technology Officer                                    ola.tuvesson@nxbio.com